| FⅪa

Factor XI, or plasma thromboplastin antecedent, is the zymogen form of factor XIa, one of the enzymes involved in coagulation. Like many other coagulation factors, it is a serine protease.Factor XIa activates factor IX by selectively cleaving arg-ala and arg-val peptide bonds. Factor IXa, in turn, forms a complex with Factor VIIIa (FIXa-FVIIIa) and activates factor X. Physiological inhibitors of factor XIa include protein Z-dependent protease inhibitor (ZPI, a member of the serine protease inhibitor/serpin class of proteins), which is independent of protein Z (its action on factor X, however, is protein Z-dependent, hence its name).
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	40	20	10	5	2.5	1.25	0.62	0
OD	2.42	1.71	1.08	0.65	0.47	0.36	0.25	0.08
Corrected OD	2.34	1.63	1	0.57	0.39	0.28	0.17	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human FⅪa spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Plasma(n=8)	92-106	102

 

| Linearity

Samples were spiked with high concentrations of Human FⅪa and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Plasma(n=4)
1 : 2	Range (%)	88-96
	Average (%)	93
1 : 4	Range (%)	87-108
	Average (%)	97